The live guides for Old Town Trolley Tours pack almost 500 years of city history into their narrated tours of St. Augustine's historic city center. Each tour includes 100 points of interest and 22 stops.

Tickets provide all-day access to the trolley and guests can disembark for shopping, dining, visiting attractions, and exploring the city on their own. Trolleys come by each stop every 15 to 20 minutes.

For visitors with disabilities:
- All trolley vehicles are equipped with ramps and lifts that can accommodate one scooter or wheelchair. Guests needing wheelchair access for loading and unloading are limited to five trolley stops along the tour route to maximize safety and ADA compliance.
- Guests with hearing impairments can receive a tour script guide upon request.
- Service dogs, as defined by the ADA, are allowed.